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KMID : 0381920070370030175
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Korean Journal of Microscopy
2007 Volume.37 No. 3 p.175 ~ p.183
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Improved Ultrastructural Preservation of Retinal Cells in Drosophila melanogaster
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Moon Ji-Young
Park Se-Jin
Han Sung-Sik
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Abstract
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The Drosophila retinal cell is widely used to study cell development and cell signaling processes. In the past decades, conventional chemical fixation had been used to study the structure of retinal cells in Drosophila. Rapid freezing methods are superior to chemical fixation methods due to their fixation speed. Some Drosophila tissues, such as the eyes, should not be freezed due to their surrounding cuticle layer. Therefore, in the case of the Drosophila retina, the benefits of high pressure freezing and freeze substitution (HPF-FS) had not been verified. In this study, a retinal cell from Drosophila melanogaster had been studied by using the HPF-FS method. Compared to chemical fixation, the preservation of the cytoplasm in the HPF-FS sample was improved on the whole. The HPF-FS cell membranes were smoother than that of chemical fixation. In addition, HPF-FS preserved the mitochondria structures very well. These results of the present study suggest that HPF-FS is superior to other fixation methods for the preservation of the retinal cell structure.
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KEYWORD
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Drosophila melanogaster, High pressure freezing-freeze substitution, Retinal cell
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